Protein Purification Reagents and Kits
STEMCELL Technologies EasySep™ RBC Depletion Reagent for RoboSep, For processing: 100 mL whole blood processed
The EasySep™ RBC Depletion Reagent is designed to immunomagnetically deplete red blood cells (RBCs) from fresh human peripheral blood, umbilical cord blood, bone marrow or leukapheresis samples by negative selection. RBCs are targeted with Tetrameric Antibody Complexes recognizing Glycophorin A and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Unwanted cells remain in the tube while desired cells are poured off.This product replaces the EasySep™ Human Glycophorin A Depletion Kit (Catalog #18352) for even faster cell isolations.
STEMCELL Technologies RoboSep™ Mouse CD117 (cKIT) Positive Selection Kit with Filter Tips, For processing: 2 x 10^9 cells
The EasySep™ Mouse CD117 (cKIT) Positive Selection Kit is designed to isolate CD117+ cells from single-cell suspensions of bone marrow by positive selection. The cocktail contains a combination of PE-labeled antibodies that is directed against CD117, which are then recognized by antibody complexes that are directed against PE and dextran. Labeled cells are bound to magnetic particles and separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.
STEMCELL Technologies EasySep™ Human PSC-Derived Cardiomyocyte Enrichment Kit, For processing: 2.5 x 10^8 cells
The EasySep™ Human PSC-Derived Cardiomyocyte Enrichment Kit is designed to isolate human PSC-derived cardiomyocytes from cell cultures by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing non-cardiomyocytes and dextran-coated magnetic particles. The labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.
STEMCELL Technologies RoboSep™ HLA Chimerism Whole Blood CD66b Positive Selection Kit, For processing: 60 mL whole blood processed
The EasySep™ HLA Chimerism Whole Blood CD66b Positive Selection Kit is designed to isolate highly purified CD66b+ cells from fresh whole blood by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD66b and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD66b antigen is expressed on mature granulocytes. Isolated cells are immediately available for downstream applications such as flow cytometry, culture, or DNA/RNA extraction for lineage-specific chimerism analysis.This product replaces the EasySep™ Human Whole Blood CD66b Positive Selection Kit (Catalog #18682) for even faster cell isolations.
![Biolegend Brilliant Violet 421™ anti-human GM-CSF [BVD2-21C11]; Isotype: Rat IgG2a κ; Reactivity: Human; Apps: ICFC; Size: 100 tests](https://assets.fishersci.com/TFS-Assets/CCG/product-images/502557566.png-250.jpg)
Biolegend Brilliant Violet 421™ anti-human GM-CSF [BVD2-21C11]; Isotype: Rat IgG2a κ; Reactivity: Human; Apps: ICFC; Size: 100 tests
Brilliant Violet 421™ anti-human GM-CSF [BVD2-21C11]; Isotype: Rat IgG2a κ; Reactivity: Human; Apps: ICFC; Size: 100 tests
Hampton Research HR2-982-25/PEGRx 1 #25 (C1), 185 ml - 0.1 M Sodium citrate tribasic dihydrate pH 5.0, 30% v/v Jeffamine ED-2001 pH 7.0
HR2-982-25/PEGRx 1 #25 (C1), 185 ml - 0.1 M Sodium citrate tribasic dihydrate pH 5.0, 30% v/v Jeffamine ED-2001 pH 7.0. Please note items from this supplier are non-returnable.
Abcam Nuclear Extraction Kit - Soluble / Insoluble Fractions
Nuclear Extraction Kit - Soluble / Insoluble Fractions
The product is subject to the following: Abcam Restricted Use Statement
STEMCELL Technologies RoboSep™ Mouse SCA1 Positive Selection Kit with Filter Tips, For processing: 2 x 10^9 cells
The EasySep™ Mouse SCA1 Positive Selection Kit is designed to isolate SCA1+ cells from single-cell suspensions of bone marrow by positive selection. The cocktail contains a combination of PE-labeled antibodies that is directed against SCA1, which are then recognized by antibody complexes that are directed against PE and dextran. Labeled cells are bound to magnetic particles and separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.This kit replaces the EasySep™ Mouse SCA1 Biotin Positive Selection Kit (Catalog #18856) for even faster cell isolations.
STEMCELL Technologies RosetteSep™ Human Cord Blood Debulking Cocktail, For processing: 200 mL cord blood processed
The RosetteSep™ Human Cord Blood Debulking Cocktail is designed to deplete lineage positive cells from fresh cord blood. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing CD2, CD14, CD19, CD66b and glycophorin A on red blood cells (RBCs). When centrifuged over a buoyant density medium such as Lymphoprep™ (Catalog #07801), the unwanted cells pellet along with the RBCs. The hematopoietic stem and progenitor cells are present as a pre-enriched population at the interface between the plasma and the buoyant density medium.
STEMCELL Technologies RoboSep™ Human Resting CD4+ T Cell Isolation Kit, For processing: 1 x 10^9 cells
The EasySep™ Human Resting CD4+ T Cell Isolation Kit is designed to isolate resting CD4+ T cells from fresh or previously frozen human peripheral blood mononuclear cells or washed leukapheresis samples by immunomagnetic negative selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically labeled cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube.
STEMCELL Technologies EasySep™ Human Central and Effector Memory CD4+ T Cell Isolation Kit, For processing: 1 x 10^9 cells
This kit isolates human CD62L+CD45RO+ and CD62L-CD45RO+ memory CD4+ T cells from fresh peripheral blood mononuclear cells (PBMCs) or washed leukapheresis samples. First, CD62L+ cells are isolated by column-free immunomagnetic positive selection using antibody complexes and EasySep™ Releasable RapidSpheres™. Then, bound magnetic particles are removed from the EasySep™-isolated CD62L+ cells, and unwanted CD45RA+ and non-CD4+ T cells are targeted for depletion using antibody complexes and EasySep™ Dextran RapidSpheres™. The final isolated fraction contains highly purified CD62L+CD45RO+ central memory CD4+ T cells that are immediately ready for downstream applications. Effector memory CD4+ T cells can be isolated from the CD62L- fraction.
STEMCELL Technologies RoboSep™ Mouse Naïve CD8+T Cell Isolation Kit, For processing: 1 x 10^9 cells
The EasySep™ Mouse Naïve CD8+ T Cell Isolation Kit is designed to isolate naïve CD62L+CD44-CD8+ T cells from single-cell suspensions of splenocytes or lymph nodes by negative selection. Unwanted cells are targeted for removal with biotinylated antibodies that are directed against non-naïve CD8+ T cells (CD4, CD11b, CD11c, CD19, CD44, CD45R/B220, CD49b, TCRγ/δ, TER119) and streptavidin-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.
New England Biolabs, Inc. NEBExpress Ni Resin – 25 ml
NEBExpress Ni Resin is an affinity matrix for the isolation and purification of polyhistidine-tagged (His-tagged) fusion proteins. It is intended for use in gravity or pressure flow columns, and batch purifications. NEBExpress Ni Resin is comprised of a highly uniform and stable chemical-tolerant resin, pre-charged with nickel ions on the matrix surface. It is resistant to a wide range of chemicals, including NaOH, EDTA, DTT and beta-Mercaptoethanol.
STEMCELL Technologies RoboSep™ Human Biotin Positive Selection Kit II, For processing: 1 x 10^9 cells
The EasySep™ Human Biotin Positive Selection Kit II is designed to isolate cells that are labeled with biotinylated antibodies. Desired cells are targeted with antibody complexes recognizing biotin and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.This product replaces the EasySep™ Human Biotin Positive Selection Kit (Catalog #18553). For highly purified particle-free cells, we recommend the EasySep™ Release Human Biotin Positive Selection Kit (Catalog #17653). (https://www.stemcell.com/products/easysep-release-human-biotin-positive-selection-kit.html)